Spherical aberration cancellation in a polarized photon-pairs confocal laser scanning microscope

Chi-Hui Chang; Chien Chou; Hsiu-Fong Chang; Hon-Fai Yau; Huan-Jang Huang; Wen-Chuan Kuo

doi:10.1117/12.728593

12 July 2007 Spherical aberration cancellation in a polarized photon-pairs confocal laser scanning microscope

Chi-Hui Chang, Chien Chou, Hsiu-Fong Chang, Hon-Fai Yau, Huan-Jang Huang, Wen-Chuan Kuo

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 66300N (2007) https://doi.org/10.1117/12.728593
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Refractive-index mismatch in conventional confocal microscopy produces severe degradation on axial resolution of sectioning image because the spherical aberration is generated in specimen. In this study, we propose a polarized photon-pairs confocal laser scanning microscope (PCLSM) in which a two-frequency linear polarized photon-pairs (LPPPs) laser beam is produced. The common-path propagation of LPPPs integrated with optical heterodyne technique not only can reduce the spherical aberration but also decreases scattering effect in specimen at same time. Therefore, the better axial and lateral resolutions of the sectioning image are produced simultaneously. In the experiment, a verification and comparison between PCLSM and conventional confocal laser scanning microscope (CLSM) on the ability of cancellation of spherical aberration induced by cover glass are demonstrated experimentally. Finally, the ability of PCLSM which can decrease the spherical aberration based on the common-path propagation of LPPPs associated with polarization gating, spatial coherence gating and spatial filtering gating is discussed.

Citation Download Citation

Chi-Hui Chang, Chien Chou, Hsiu-Fong Chang, Hon-Fai Yau, Huan-Jang Huang, and Wen-Chuan Kuo "Spherical aberration cancellation in a polarized photon-pairs confocal laser scanning microscope", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300N (12 July 2007); https://doi.org/10.1117/12.728593

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available