Paper
23 July 2007 In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin
Ronan Le Harzic, Anne Colonna, Rainer Bückle, Alexander Ehlers, Christophe Hadjur, Frederic Leroy, Frederic Flament, Roland Bazin, Bertrand Piot, Iris Riemann, Karsten König
Author Affiliations +
Abstract
In vivo simultaneous collagen and elastin measurements using the multiphoton tomograph DermaInspect have been performed in skin dermis. We showed it was possible to get simultaneous measurements of autofluorescence (AF) and Second Harmonic Generation (SHG) with a newly developed device using 2 PMTs for time-correlated single photon counting. Unlike elastin (AF), collagen structures are able to generate second harmonics (SHG). Comparing the images and SHG / AF ratios recorded in the depth of the outer and inner sides of the forearm of two European female volunteers (31 and 60 years old, respectively) shows differences in collagen and elastin fibres density. It decreases with depth in the 60 years old volunteer compared to the younger one, and the skin of younger volunteer shows more collagen.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ronan Le Harzic, Anne Colonna, Rainer Bückle, Alexander Ehlers, Christophe Hadjur, Frederic Leroy, Frederic Flament, Roland Bazin, Bertrand Piot, Iris Riemann, and Karsten König "In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300V (23 July 2007); https://doi.org/10.1117/12.727044
Lens.org Logo
CITATIONS
Cited by 1 scholarly publication.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Atrial fibrillation

Second-harmonic generation

Collagen

Skin

In vivo imaging

Harmonic generation

Multiphoton tomography

RELATED CONTENT


Back to Top