Portable multi-immunosensing lab-on-a-chip (LOC) triggered by air bladder

Tae Ho Kang; Sin Wook Park; Jun Hwang Lee; Hyun C. Yoon; Sang Sik Yang

doi:10.1117/12.759333

27 December 2007 Portable multi-immunosensing lab-on-a-chip (LOC) triggered by air bladder

Tae Ho Kang, Sin Wook Park, Jun Hwang Lee, Hyun C. Yoon, Sang Sik Yang

Proceedings Volume 6799, BioMEMS and Nanotechnology III; 67991C (2007) https://doi.org/10.1117/12.759333
Event: SPIE Microelectronics, MEMS, and Nanotechnology, 2007, Canberra, ACT, Australia

Abstract

This paper presents a simple and reliable multi-immunosensing lab-on-a-chip detecting antibodies as multi-disease markers using electrochemical method suitable for a portable point-of-care system. Since multi-immunosensing LOCs are to be disposable and cheap, the complications associated with the liquid control need to be removed. The main complication arises from the active microfluidic part driven by the external electric power. In this paper, a multi-stacked PDMS LOC including PDMS passive valves is proposed. The sequential liquid driving by capillary attraction and the action of check valve provide a reliable immunosensing tool simply triggered by an air bladder push without an electrical power. The immunosensing-LOC with the size of "25mm × 20mm × 6 mm" is fabricated with PDMS using the replica molding and oxygen plasma bonding. The LOC consists of a PDMS valve, channel, and a glass substrate. The fluidic tests were performed using DI water. The liquids are controlled by two kinds of passive valve, one is capillary stop valve and the other is membrane type check valve. The capillary stop valve stops liquids using pressure barrier of expanded channel. The check valve stops the liquid triggered by an air bladder from flowing backward. The assembly of these two valves assures the well controlled liquid driving for the immunosensing. The model experiments were performed with anti-DNP antibody and anti-biotin antibody as target analytes. The antibodies conjugated with GOX are used as a signaling molecule for cyclic voltammetry. The different amplified signals show different target analyte affinities and make sure the multi-immunosensing.

Citation Download Citation

Tae Ho Kang, Sin Wook Park, Jun Hwang Lee, Hyun C. Yoon, and Sang Sik Yang "Portable multi-immunosensing lab-on-a-chip (LOC) triggered by air bladder", Proc. SPIE 6799, BioMEMS and Nanotechnology III, 67991C (27 December 2007); https://doi.org/10.1117/12.759333

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available