Simple micropatterning of proteins using polyelectrolyte multilayers and microcontact printing

Ji-Hye Lee; Chang-Hyung Choi; Chang-Soo Lee

doi:10.1117/12.768573

27 December 2007 Simple micropatterning of proteins using polyelectrolyte multilayers and microcontact printing

Ji-Hye Lee, Chang-Hyung Choi, Chang-Soo Lee

Proceedings Volume 6799, BioMEMS and Nanotechnology III; 67991F (2007) https://doi.org/10.1117/12.768573
Event: SPIE Microelectronics, MEMS, and Nanotechnology, 2007, Canberra, ACT, Australia

Abstract

The selective immobilization of various biomolecules in well-defined area is important technique for the development of biosensors and biochips. Especially, the fabrication of protein micropatterns preserving their functional activity on the desired surface is critical issue for the development of medical diagnostic devices and basic protein studies. In this study, we have introduced a simple but reliable method of protein patterning on functionalized polyelectrolyte thin films (PEL) through consecutive layer-by-layer adsorption of polyelectrolytes via self-assembly technique and microcontact printing (μCP). For the selection of appropriate surface, several representative surfaces modified with various functional materials including aldehyde, epoxide, poly-L-lysine, amine, and self-assembled polyelectrolyte multilayers (PEL) were investigated. The PEL surface providing electrostatic interaction force showed most high functionality in point of homogeneous patterning of proteins with high density and preservation of inherent 3-dimensional structure of proteins. Immunoassay as a model system of protein-protein interaction showed good linearity, indicating the feasibility of a quantitative measurement of the concentration of target proteins in sample. Our proposed approach based on PEL constructed by self-assembly technique in aqueous solution is green chemistry and cost-effective method to generate stable 3-D thin film on surface. The demand for strict control over the positioning and the stable immobilization of several kinds of biomolecules in fabricated structures can result in many applications.

Citation Download Citation

Ji-Hye Lee, Chang-Hyung Choi, and Chang-Soo Lee "Simple micropatterning of proteins using polyelectrolyte multilayers and microcontact printing", Proc. SPIE 6799, BioMEMS and Nanotechnology III, 67991F (27 December 2007); https://doi.org/10.1117/12.768573

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Proteins

Luminescence

Printing

Glasses

Multilayers

Optical lithography

Adsorption

Show All Keywords

Keywords/Phrases

Search In:

Publication Years