Mechanisms of light scattering from biological cells and cell culture

Tao T. Wu; Jianan Y. Qu

doi:10.1117/12.778579

8 January 2008 Mechanisms of light scattering from biological cells and cell culture

Tao T. Wu, Jianan Y. Qu

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68260P (2008) https://doi.org/10.1117/12.778579
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

The mechanisms of light scattering from biological cells are investigated by using confocal microscopy and light scattering spectroscopy (LSS). The LSS system measures the light scattering in the visible wavelength range from 1.1° to 165.0°. The results provide evidence that the small-sized subcellular structures are the major contributors to the backscattering signals. A unified Mie and fractal model is proposed to interpret light scattering by biological cells. The results demonstrate that Mie scattering from bare cells and nuclei is dominant in small forward scattering angles. But Mie scattering from bare cells and nuclei is found not to be able to provide a satisfactory interpretation of scattering spectral signals in the large angles, which is determined by fractal scattering from the subcellular structures. The findings of the theoretical model are consistent with the results of experimental investigation on the light scattering from biological cells.

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Tao T. Wu and Jianan Y. Qu "Mechanisms of light scattering from biological cells and cell culture", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68260P (8 January 2008); https://doi.org/10.1117/12.778579

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KEYWORDS

Light scattering

Scattering

Mie scattering

Fractal analysis

Refractive index

Confocal microscopy

Backscatter

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