The effect of oxygen on the photobleaching of hematoporphrin monomethyl ether (HMME) in different solutions

Y. Wang; Y. Gu; F. G. Liu; N. Y. Huang

doi:10.1117/12.755798

8 January 2008 The effect of oxygen on the photobleaching of hematoporphrin monomethyl ether (HMME) in different solutions

Y. Wang, Y. Gu, F. G. Liu, N. Y. Huang

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68261N (2008) https://doi.org/10.1117/12.755798
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

To analyse the effect of oxygen on the photobleaching of HMME, photobleaching rates of HMME when bubbled with oxygen or argon, added with singlet oxygen quenchers or hydroxyl radical quencher were compared. According to the solvents in which HMME was dissolved, four groups were set, HMME-DMSO, HMME-PBS, HMME-Albumin buffer, HMME-Cells suspension. Each group was divided into four subgroups, bubbled with oxygen or argon, added with singlet oxygen quenchers or hydroxyl radical quencher respectively. Spectra measurement was performed at 10min intervals for a period of 40 min before and after laser irradiation, photobleaching rates under different condition were compared. Photobleaching was the fastest when bubbled with oxygen, apparentely inhibited when bubbled with argon or added with singlet oxygen quenchers. Hydroxyl radical quencher can inhibit photopleaching of HMME slightly, but its inhibition was gentler than that of singlet oxygen quenchers and argon. Oxygen plays an inportant role in thre photobleaching of HMME, and the process is singlet oxygen mediated self-sensitized photooxidation.

Citation Download Citation

Y. Wang, Y. Gu, F. G. Liu, and N. Y. Huang "The effect of oxygen on the photobleaching of hematoporphrin monomethyl ether (HMME) in different solutions", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68261N (8 January 2008); https://doi.org/10.1117/12.755798

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available