Three dimensional biological microscopic image restoration with adaptive local regularization parameter based on wavelet domain

Hua Chen; Fuying Huang

doi:10.1117/12.755387

11 January 2008 Three dimensional biological microscopic image restoration with adaptive local regularization parameter based on wavelet domain

Hua Chen, Fuying Huang

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68262C (2008) https://doi.org/10.1117/12.755387
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

A method is proposed for adaptively choosing local regularization parameter based on gray scale difference estimation, and used to three-dimensional (3D) biological microscopic image restoration MPMAP algorithm. Every optical-sectioning image of 3D microscopic image with noise is decomposed in wavelet domain, and then its high frequency images of horizontal , vertical and diagonal direction are reconstructed. Then the images are convoluted respectively with corresponding direction operator, and the local gray scale differences of high frequency images before and after convolution are calculated. The minimum of corresponding local gray scale differences in the high frequency images is selected as local gray scale difference estimations of the optical-sectioning image, then the local regularization parameter of the optical-sectioning image is chosen by mapping local gray scale difference estimation. The local regularization parameter of the 3D microscopic image is made of the local regularization parameters of every optical-sectioning image. The test results show that the local regularization parameter based on gray scale difference estimation can describe more accurately intensity and position of noises than noise variance estimation. The local regularization parameter is used to 3D biological microscopic image regularization restoration with MPMAP algorithm. Experimental results show that better super-resolution effect is reached than whole regularization parameter MPMAP.

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Hua Chen and Fuying Huang "Three dimensional biological microscopic image restoration with adaptive local regularization parameter based on wavelet domain", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68262C (11 January 2008); https://doi.org/10.1117/12.755387

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KEYWORDS

Image restoration

3D image processing

3D image reconstruction

Signal to noise ratio

Wavelets

3D image restoration

Biological research

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