Rapid and quantitative measuring of telomerase activity using an electro-chemiluminescent sensor

Xiaoming Zhou; Da Xing; Debin Zhu; Li Jia

doi:10.1117/12.760222

26 November 2007 Rapid and quantitative measuring of telomerase activity using an electrochemiluminescent sensor

Xiaoming Zhou, Da Xing, Debin Zhu, Li Jia

Proceedings Volume 6830, Advanced Sensor Systems and Applications III; 68300L (2007) https://doi.org/10.1117/12.760222
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Telomerase, a ribonucleoprotein enzyme that adds telomeric repeats to the 3'end of chromosomal DNA for maintaining chromosomal integrity and stability. This strong association of telomerase activity with tumors establishing it is the most widespread cancer marker. A number of assays based on the polymerase chain reaction (PCR) have been developed for the evaluation of telomerase activity. However, those methods require gel electrophoresis and some staining procedures. We developed an electrochemiluminescent (ECL) sensor for the measuring of telomerase activity to overcome these problems such as troublesome post-PCR procedures and semi-quantitative assessment in the conventional method. In this assay 5'-biotinylated telomerase synthesis (TS) primer serve as the substrate for the extension of telomeric repeats under telomerase. The extension products were amplified with this TS primer and a tris-(2'2'-bipyridyl) ruthenium (TBR)-labeled reversed primer. The amplified products was separated and enriched in the surface of electrode by streptavidin-coated magnetic beads, and detected by measuring the ECL signals of the TBR labeled. Measuring telomerase activity use the sensor is easy, sensitive, rapid, and applicable to quantitative analysis, should be clinically useful for the detection and monitoring of telomerase activity.

Citation Download Citation

Xiaoming Zhou, Da Xing, Debin Zhu, and Li Jia "Rapid and quantitative measuring of telomerase activity using an electrochemiluminescent sensor", Proc. SPIE 6830, Advanced Sensor Systems and Applications III, 68300L (26 November 2007); https://doi.org/10.1117/12.760222

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