Photodynamic therapy of otitis media in-vitro and in-vivo using gerbil

Chung-Ku Rhee M.D.; Pil Seung Kwon; Jin Chul Ahn; Phil Sang Chung; Ruifeng Ge

doi:10.1117/12.774983

8 February 2008 Photodynamic therapy of otitis media in-vitro and in-vivo using gerbil

Chung-Ku Rhee M.D., Pil Seung Kwon, Jin Chul Ahn, Phil Sang Chung, Ruifeng Ge

Proceedings Volume 6842, Photonic Therapeutics and Diagnostics IV; 68422Y (2008) https://doi.org/10.1117/12.774983
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

The aim of this study was to evaluate antibacterial effects of PDT on common bacteria causing otitis media with effusion (OME). In vitro study was carried out using a hematoporphyrin derivative sensitizer (photogem) and 632 nm diode laser on H. influenzae, M. catarrhalis, and S. pneumoniae. One ml of each bacterial suspension was incubated for 3 hours and various concentrations of photogem were administered into the suspension. The suspensions were irradiated with 632 diode laser (15 J/cm²). The presence of colony forming units of the bacteria was examined, microscopic structures of bacteria were examined by TEM, and cytometry of bacteria was performed. The PDT was effective in killing all 3 kinds of bacteria. TEM showed damaged bacterial cell membrane and cytoplasmic structures and the flow cytometry showed lower number of viable bacteria in PDT group comparing to the control group. In vivo PDT study was performed using gerbil. S. pneumoniae or H. influenzae was injected into bullae. Photogem was injected into bullae in 2 days by when OME was developed and transcanal irradiation of 632 nm diode laser (90 J) was performed with a fiber perforated through an ear drum into a middle ear cavity and bulla. Four days after PDT, middle ear and bulla were washed with DPBS and the washed DPBS was cultured. The presence of bacterial colonies was examined. PDT was effective in killing S. pneumoniae in 87 % of the infected bullae with OME while it was effective to eradicate H. influenzae in 50 % of the infected bullae with OME. The results of these studies demonstrated that PDT may be effective to treat otitis media. It may have clinical implication to treat otitis media that is resistant to antibiotic therapy.

Citation Download Citation

Chung-Ku Rhee M.D., Pil Seung Kwon, Jin Chul Ahn, Phil Sang Chung, and Ruifeng Ge "Photodynamic therapy of otitis media in-vitro and in-vivo using gerbil", Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422Y (8 February 2008); https://doi.org/10.1117/12.774983

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KEYWORDS

Photodynamic therapy

Bacteria

Ear

Picosecond phenomena

In vitro testing

Transmission electron microscopy

In vivo imaging

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