Using contrast transfer function to evaluate the effect of motion blur on microscope image quality

Marc C. Wood; Xingwei Wang; Bin Zheng; Shibo Li; Wei Chen; Hong Liu

doi:10.1117/12.759203

18 February 2008 Using contrast transfer function to evaluate the effect of motion blur on microscope image quality

Marc C. Wood, Xingwei Wang, Bin Zheng, Shibo Li, Wei Chen, Hong Liu

Proceedings Volume 6857, Biophotonics and Immune Responses III; 68570F (2008) https://doi.org/10.1117/12.759203
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Scanning of microscope slides is an important part of cytogenetic diagnosis. Metaphase chromosomes arranged in a karyotype reveal the nature and severity of cancer and other diseases. Searching for metaphases spreads is a lengthy and tedious process that can benefit from computer aided systems. When slides are searched by such systems in continuous motion, the image quality is reduced. The motion blur is a function of the scan speed, the camera frame rate and sample time, and the level of magnification. In this study, normalized contrast transfer function (CTF) is used to define the amount of image degradation.

Citation Download Citation

Marc C. Wood, Xingwei Wang, Bin Zheng, Shibo Li, Wei Chen, and Hong Liu "Using contrast transfer function to evaluate the effect of motion blur on microscope image quality", Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570F (18 February 2008); https://doi.org/10.1117/12.759203

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