Three-dimensional quantitative phase imaging: current and future perspectives

Nicoleta M. Dragomir; Xiao Ming Goh; Ann Roberts

doi:10.1117/12.762619

12 February 2008 Three-dimensional quantitative phase imaging: current and future perspectives

Nicoleta M. Dragomir, Xiao Ming Goh, Ann Roberts

Proceedings Volume 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV; 686106 (2008) https://doi.org/10.1117/12.762619
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Great effort has been made in the recent past to develop new non-destructive imaging modalities for both two and three dimensional objects, based on the phase properties of a specimen. Quantitative phase tomography (QPT) is a hybrid technique that has been proposed to provide three-dimensional (3D) refractive index (RI) profiling of irregular phase objects by combining transverse phase measurements with traditional tomographic reconstruction techniques. This profiling is accomplished through measurements of sets of projections which are ultimately related to the RI values of the object's transverse cross-section. This is particularly useful for 3D refractive index determination of specimens where staining is not appropriate or for materials that cannot be stained and is essential to many applications in photonics and biotechnology. This article reviews recent developments in quantitative phase tomography as they are presently available and suggests future applications based on current research on the 3D RI. The enabling elements for 3D QPT in the context of four key areas are discussed: the effect of the refractive index of the surrounding matching fluid, spatial resolution, phase accuracy and optimal defocus. Recent progress and future perspectives related to each of these areas is presented with regard to various test objects of known optical properties.

Citation Download Citation

Nicoleta M. Dragomir, Xiao Ming Goh, and Ann Roberts "Three-dimensional quantitative phase imaging: current and future perspectives", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686106 (12 February 2008); https://doi.org/10.1117/12.762619

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