Enhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents

Adam de la Zerda; Zhuang Liu; Cristina Zavaleta; Sunil Bodapati; Robert Teed; Srikant Vaithilingam; Te-Jen Ma; Omer Oralkan; Xiaoyuan Chen; Butrus T. Khuri-Yakub; Hongjie Dai; Sanjiv S. Gambhir

doi:10.1117/12.809601

24 February 2009 Enhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents

Adam de la Zerda, Zhuang Liu, Cristina Zavaleta, Sunil Bodapati, Robert Teed, Srikant Vaithilingam, Te-Jen Ma, Omer Oralkan, Xiaoyuan Chen, Butrus T. Khuri-Yakub, Hongjie Dai, Sanjiv S. Gambhir

Author Affiliations +

Proceedings Volume 7177, Photons Plus Ultrasound: Imaging and Sensing 2009; 71772K (2009) https://doi.org/10.1117/12.809601
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Photoacoustic imaging of living subjects offers high spatial resolution at increased tissue depths compared to purely optical imaging techniques. We have recently shown that intravenously injected single walled carbon nanotubes (SWNTs) can be used as targeted photoacoustic imaging agents in living mice using RGD peptides to target α_vβ₃ integrins. We have now developed a new targeted photoacoustic imaging agent based on SWNTs and Indocyanine Green (SWNT-ICG) with absorption peak at 780nm. The photoacoustic signal of the new imaging agent is enhanced by ~20 times as compared to plain SWNTs. The particles are synthesized from SWNT-RGD that noncovalently attach to multiple ICG molecules through pi-pi stacking interactions. Negative control particles had RAD peptide instead of RGD. We measured the serum stability of the particles and verified that the RGD/RAD conjugation did not alter the particle's absorbance spectrum. Finally, through cell uptake studies with U87MG cells we verified that the particles bind selectively to α_vβ₃ integrin. In conclusion, the extremely high absorption of the SWNT-ICG particles shows great promise for high sensitivity photoacoustic imaging of molecular targets in-vivo. This work lays the foundations for future in-vivo studies that will use the SWNT-ICG particles as imaging agents administered systemically.

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Adam de la Zerda, Zhuang Liu, Cristina Zavaleta, Sunil Bodapati, Robert Teed, Srikant Vaithilingam, Te-Jen Ma, Omer Oralkan, Xiaoyuan Chen, Butrus T. Khuri-Yakub, Hongjie Dai, and Sanjiv S. Gambhir "Enhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents", Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71772K (24 February 2009); https://doi.org/10.1117/12.809601

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KEYWORDS

Photoacoustic spectroscopy

Single walled carbon nanotubes

Particles

Absorption

Photoacoustic imaging

Transducers

Tissue optics

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