Involvement of caspase-dependent and -independent apoptotic pathways in cisplatin-induced apoptosis

Lei Liu; Yingjie Zhang; Xianwang Wang

doi:10.1117/12.808431

24 February 2009 Involvement of caspase-dependent and -independent apoptotic pathways in cisplatin-induced apoptosis

Lei Liu, Yingjie Zhang, Xianwang Wang

Proceedings Volume 7178, Biophotonics and Immune Responses IV; 71780N (2009) https://doi.org/10.1117/12.808431
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Cisplatin, an efficient anticancer agent, can trigger multiple apoptotic pathways in cancer cells. However, the signal transduction pathways in response to cisplatin-based chemotherapy are complicated, and the mechanism is not fully understood. In current study, we showed that, during cisplatin-induced apoptosis of human lung adenocarcinoma cells, both the caspase-dependent and -independent pathways were activated. Herein, we reported that after cisplatin treatment, the activities of caspase-9/-3 were sharply increased; pre-treatment with Z-LEHD-fmk (inhibitor of caspase-9), Z-DEVD-fmk (inhibitor of caspase-3), and Z-VAD-fmk (a pan-caspase inhibitor) increased cell viability and decreased apoptosis, suggesting that caspase-mediated apoptotic pathway was activated following cisplatin treatment. Confocal imaging of the cells transfected with AIF-GFP demonstrated that AIF release occurred about 9 h after cisplatin treatment. The event proceeded progressively over time, coinciding with a nuclear translocation and lasting for more than 2 hours. Down-regulation of AIF by siRNA also significantly increased cell viability and decreased apoptosis, these results suggested that AIF-mediated caspase-independent apoptotic pathway was involved in cispatin-induced apoptosis. In conclusion, the current study demonstrated that both caspase-dependent and -independent apoptotic pathways were involved in cisplatin-induced apoptosis in human lung adenocarcinoma cells.

Citation Download Citation

Lei Liu, Yingjie Zhang, and Xianwang Wang "Involvement of caspase-dependent and -independent apoptotic pathways in cisplatin-induced apoptosis", Proc. SPIE 7178, Biophotonics and Immune Responses IV, 71780N (24 February 2009); https://doi.org/10.1117/12.808431

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