In vivo optical analysis of pancreatic cancer tissue in living model mice using fluorescence and Raman spectroscopic techniques

Toshiaki Suzuki; Yusuke Hattori; Takashi Katagiri; Hiroki Mitsuoka; Ken-ichi Sato; Toru Asakura; Toru Shimosegawa; Hidetoshi Sato

doi:10.1117/12.808832

12 February 2009 In vivo optical analysis of pancreatic cancer tissue in living model mice using fluorescence and Raman spectroscopic techniques

Toshiaki Suzuki, Yusuke Hattori, Takashi Katagiri, Hiroki Mitsuoka, Ken-ichi Sato, Toru Asakura, Toru Shimosegawa, Hidetoshi Sato

Author Affiliations +

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 718202 (2009) https://doi.org/10.1117/12.808832
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Living pancreatic cancer tissues grown subcutaneously in nude mice are studied by in vivo Raman spectroscopy and autofluorescence imaging. Comparing the same point spectra of alive pancreatic cancer tissue to that of the dead tissue, it is found that they are different each other. The results suggest that the spectral changes reflect the protein conformational changes in the tumor tissue with death of the host animal. From the result of autofluorescence study, in vivo autofluorescence imaging has potential as a method to assign the histological elements of the pancreatic cancer tissue without any staining. These results strongly suggest that combination of these techniques is very important to study biological tissue.

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Toshiaki Suzuki, Yusuke Hattori, Takashi Katagiri, Hiroki Mitsuoka, Ken-ichi Sato, Toru Asakura, Toru Shimosegawa, and Hidetoshi Sato "In vivo optical analysis of pancreatic cancer tissue in living model mice using fluorescence and Raman spectroscopic techniques", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718202 (12 February 2009); https://doi.org/10.1117/12.808832

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