Dynamic reconfigurable spectral imaging microscopy

Bing Chen; Jame J. Yang; Michael R. Wang

doi:10.1117/12.810548

12 February 2009 Dynamic reconfigurable spectral imaging microscopy

Bing Chen, Jame J. Yang, Michael R. Wang

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71821D (2009) https://doi.org/10.1117/12.810548
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.

Citation Download Citation

Bing Chen, Jame J. Yang, and Michael R. Wang "Dynamic reconfigurable spectral imaging microscopy", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821D (12 February 2009); https://doi.org/10.1117/12.810548

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