A two-stage morphological classifier of foci occurring in cell transformation assays

Giovanni F. Crosta; Chiara Urani; Luca Bussinelli

doi:10.1117/12.808837

23 February 2009 A two-stage morphological classifier of foci occurring in cell transformation assays

Giovanni F. Crosta, Chiara Urani, Luca Bussinelli

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71821M (2009) https://doi.org/10.1117/12.808837
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Cell Transformation Assays (CTA) rely on the detection of phenotypic changes, namely foci, induced by chemicals (e.g., xenobiotics or candidate drugs) in mammalian cells such as C3H10T1/2 mouse fibroblasts. A focus is a cell colony and as such is made visible by standardized techniques of light microscopy. Foci exhibit a variety of morphological features, by which three "Types" have been defined. Types II and III consist of cells having undergone neoplastic transformation. The assignment of a focus to a Type is based on the evaluation of phenotypic features by a trained human expert. An automated, two-stage morphological classifier of foci is described herewith. Morphological descriptors are extracted from light microscope images by the "spectrum enhancement" algorithm, which separates structure from texture. Said descriptors are submitted to a classifier, the first stage of which is trained to discriminate transformed cells from normal ones and the 2nd stage to discriminate Type III from Type II. The classifier operating in recognition mode (on images not used for training) is satisfactory in terms of confusion matrix entries. The whole procedure is aimed at removing subjectivity from the scoring and classification of foci and thus make CTA a more powerful tool in carcinogenesis studies.

Citation Download Citation

Giovanni F. Crosta, Chiara Urani, and Luca Bussinelli "A two-stage morphological classifier of foci occurring in cell transformation assays", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821M (23 February 2009); https://doi.org/10.1117/12.808837

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