3D effects in DIC images of extended objects

Heidy Sierra; Charles A. DiMarzio; Dana H. Brooks

doi:10.1117/12.809836

3 March 2009 3D effects in DIC images of extended objects

Heidy Sierra, Charles A. DiMarzio, Dana H. Brooks

Proceedings Volume 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI; 71840D (2009) https://doi.org/10.1117/12.809836
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Phase microscopy has been widely used to acquire information about unstained transparent biological objects. These objects are well visualized by techniques such as Differential Interference Contrast (DIC) microscopy, but standard DIC systems don't provide quantitative phase information. Quantitative analysis is limited by the nonlinear relation between the intensity image and the object phase. In this work we combine nonlinearity concepts of the image formation process to model 3D DIC images, specifically the point spread function (PSF) formulation; capturing behavior of measured images along the optical axis that are not obtained when using other models. We verify our model by comparing simulated images to real measured images. This model is designed so that the reconstruction of the 3D properties of imaged specimens can be done in a simple form.

Citation Download Citation

Heidy Sierra, Charles A. DiMarzio, and Dana H. Brooks "3D effects in DIC images of extended objects", Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 71840D (3 March 2009); https://doi.org/10.1117/12.809836

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