Intracellular delivery of and sensing with quantum dot bioconjugates

James B. Delehanty; Christopher E. Bradburne; Igor L. Medintz; Dorothy Farrell; Thomas Pons; Jeffrey R. Deschamps; Florence M. Brunel; Philip E. Dawson; Hedi Mattoussi

doi:10.1117/12.809286

3 March 2009 Intracellular delivery of and sensing with quantum dot bioconjugates

James B. Delehanty, Christopher E. Bradburne, Igor L. Medintz, Dorothy Farrell, Thomas Pons, Jeffrey R. Deschamps, Florence M. Brunel, Philip E. Dawson, Hedi Mattoussi

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Proceedings Volume 7189, Colloidal Quantum Dots for Biomedical Applications IV; 71890W (2009) https://doi.org/10.1117/12.809286
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Luminescent semiconductor quantum dots (QDs) possess several unique optical properties that suggest they will be superior reagents compared to traditional organic fluorophores for applications such as the labeling of subcellular structures and the sensing of biological processes within living cells. Chief among these properties are their 1) high quantum yields, 2) broad absorption spectra coupled with narrow symmetric, size-tunable emissions and 3) the ability to excite multiple QD populations at a single wavelength removed from emission. This latter attribute presents the exciting possibility of multiplexed or "multicolor" intracellular imaging and sensing. In order for QDs to reach their full potential as intracellular imaging and sensing reagents, however, facile and robust methodologies for delivering QDs in a controlled manner to specific subcellular locations must be developed. We have investigated a number of strategies to achieve the intracellular delivery of QDs including peptide-mediated, polymer-mediated, and microinjection based methods. In particular, the ability to selectively deliver biofunctionalized QDs to specific intracellular compartments is being targeted. Additionally, long-term QD intracellular QD fate, stability and toxicity are also concomitantly being examined. Cellular delivery experiments utilizing these various schemes will be highlighted and the relative advantages and disadvantages of each approach will be discussed.

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James B. Delehanty, Christopher E. Bradburne, Igor L. Medintz, Dorothy Farrell, Thomas Pons, Jeffrey R. Deschamps, Florence M. Brunel, Philip E. Dawson, and Hedi Mattoussi "Intracellular delivery of and sensing with quantum dot bioconjugates", Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 71890W (3 March 2009); https://doi.org/10.1117/12.809286

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KEYWORDS

Polymers

Quantum dots

Toxicity

Luminescence

Absorption

Analytical research

Multiplexing

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