Enhanced drug transport through alginate biofilms using magnetic nanoparticles

Shayna L. McGill; Carla Cuylear; Natalie L Adolphi; Marek Osinski; Hugh Smyth

doi:10.1117/12.816830

3 March 2009 Enhanced drug transport through alginate biofilms using magnetic nanoparticles

Shayna L. McGill, Carla Cuylear, Natalie L Adolphi, Marek Osinski, Hugh Smyth

Proceedings Volume 7189, Colloidal Quantum Dots for Biomedical Applications IV; 718918 (2009) https://doi.org/10.1117/12.816830
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The development of microbiological biofilms greatly reduces the efficacy of antibiotic therapies and is a serious problem in chronic infection and for implantable medical devices. We investigated the potential of superparamagnetic nanoparticles to increase transport through in vitro models of alginate biofilms. An in vitro alginate biofilm model was developed to mimic the composition of in vivo samples of P. aeruginosa infections. Transport through this model biofilm was performed using both bulk diffusion methods and single particle tracking techniques in the presence and absence of an external magnetic field. Bulk diffusion of nanoparticles through the biofilm was significantly enhanced in the presence of a magnetic field, both visually and quantitatively. Nanoparticle trajectories also showed transport increases were significantly higher when magnetic fields were applied. We also showed that surface chemistry (cationic, anioni, or neutral) of the nanoparticles significantly influenced transport rates. Finally, nanoparticle size also influenced the transport rates and variability of transport rates through the biofilm. In these first studies using magnetic nanoparticles in bacterial biofilms, we demonstrate that transport enhancement can be achieved and further studies are warranted.

Citation Download Citation

Shayna L. McGill, Carla Cuylear, Natalie L Adolphi, Marek Osinski, and Hugh Smyth "Enhanced drug transport through alginate biofilms using magnetic nanoparticles", Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 718918 (3 March 2009); https://doi.org/10.1117/12.816830

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