Rapid line-scan confocal imaging of retinal activation

Yang-Guo Li; Xin-Cheng Yao

doi:10.1117/12.842526

2 March 2010 Rapid line-scan confocal imaging of retinal activation

Yang-Guo Li, Xin-Cheng Yao

Proceedings Volume 7550, Ophthalmic Technologies XX; 75500D (2010) https://doi.org/10.1117/12.842526
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A rapid line-scan confocal imager was developed for reflected light measurement of fast IOSs correlated with retinal activation. This functional imager provides two, i.e., frame-by-frame and line-by-line, imaging modalities. While frame-by-frame imaging allows dynamic visualization of IOSs over a two-dimensional retinal area at a frame speed > 100 Hz; line-by-line recording can provide ultrafast (> 10 KHz) monitoring of a fixed line area of the retina. A series of experiments was conducted to characterize reflected IOSs in frog retinas, and simultaneous electrophysiological responses were measured. Our experiments indicated that reflected IOSs were tightly correlated with retinal stimuli. Because of effective rejection of out-of-focus background light, rapid confocal imaging typically disclosed fast IOSs with magnitude peak > 30% ▵I/I, where ▵I was dynamic optical change and I was background light intensity. We anticipate that further development of the IOS imaging technology will pave the way toward noninvasive, high resolution evaluation of retinal function.

Citation Download Citation

Yang-Guo Li and Xin-Cheng Yao "Rapid line-scan confocal imaging of retinal activation", Proc. SPIE 7550, Ophthalmic Technologies XX, 75500D (2 March 2010); https://doi.org/10.1117/12.842526

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