LED illumination effects on proliferation and survival of meningioma cellular cultures

Efrain Solarte; Hernan Urrea; William Criollo; Oscar Gutierrez

doi:10.1117/12.843060

23 February 2010 LED illumination effects on proliferation and survival of meningioma cellular cultures

Efrain Solarte, Hernan Urrea, William Criollo, Oscar Gutierrez

Proceedings Volume 7563, Dynamics and Fluctuations in Biomedical Photonics VII; 756309 (2010) https://doi.org/10.1117/12.843060
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Meningioma cell cultures were prepared from frozen cell samples in 96 wells culture plates. Semiconductor light sources (LED) in seven different wavelength ranges were used to illuminate the wells, three different irradiation doses were selected per LED. Control cultures using three different concentrations of FBS were processed for comparison. Cell proliferation, viability, and cytotoxicity were measured every 24 hours for 6 days, using the XTT colorimetric assay (Roche^R). None of the irradiated cultures exhibit cytotoxicity; but some of them exhibit proliferation inhibition. The larger proliferation was detected at a 0.05J/cm² dose, for all LEDs; but for the orange and violet LEDs generated the bigger proliferation rate was measured. Results show the improvement of meningioma cell proliferation using illumination in some given wavelength ranges.

Citation Download Citation

Efrain Solarte, Hernan Urrea, William Criollo, and Oscar Gutierrez "LED illumination effects on proliferation and survival of meningioma cellular cultures", Proc. SPIE 7563, Dynamics and Fluctuations in Biomedical Photonics VII, 756309 (23 February 2010); https://doi.org/10.1117/12.843060

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