High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method

Youngjae Won; Donguk Kim; Wenzhong Yang; Dug Y. Kim

doi:10.1117/12.840387

24 February 2010 High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method

Youngjae Won, Donguk Kim, Wenzhong Yang, Dug Y. Kim

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 756816 (2010) https://doi.org/10.1117/12.840387
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.

Citation Download Citation

Youngjae Won, Donguk Kim, Wenzhong Yang, and Dug Y. Kim "High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756816 (24 February 2010); https://doi.org/10.1117/12.840387

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available