Nonlinear 3D microscopy of ex vivo corneas

Juan M. Bueno; Emilio J. Gualda; Pablo Artal

doi:10.1117/12.840720

26 February 2010 Nonlinear 3D microscopy of ex vivo corneas

Juan M. Bueno, Emilio J. Gualda, Pablo Artal

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756937 (2010) https://doi.org/10.1117/12.840720
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A multiphoton microscope has been developed to investigate the sources of nonlinear fluorescence (TPEF) and second harmonic generation (SHG) in non-stained samples of ex-vivo corneas. Stacks of images from different depths are recorded to reconstruct high-resolution 3D (volume) images of the cornea. The corneal epithelium and endothelium provide significant TPEF signal, while the only source of SHG is the stroma. Within the stroma, the keratocytes can also be visualized. Volumetric 3D images of the cornea combining TPEF and SHG signals are useful to characterize the organization of the corneal collagen and to describe the distribution of keratocytes. These images will help to better understand how different pathologies modify the corneal structure and to control the changes produced by surgical or healing processes.

Citation Download Citation

Juan M. Bueno, Emilio J. Gualda, and Pablo Artal "Nonlinear 3D microscopy of ex vivo corneas", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756937 (26 February 2010); https://doi.org/10.1117/12.840720

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available