Adaptive optics multiphoton microscopy

Emilio J. Gualda; Juan M. Bueno; Pablo Artal

doi:10.1117/12.845981

18 February 2010 Adaptive optics multiphoton microscopy

Emilio J. Gualda, Juan M. Bueno, Pablo Artal

Proceedings Volume 7595, MEMS Adaptive Optics IV; 75950G (2010) https://doi.org/10.1117/12.845981
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

We have developed an adaptive optics multiphoton microscope. The multiphoton imaging system combines an ultrafast high-power laser, a scanning unit, a motorized Z-scan device and a photon-counting detector. The adaptive optics module is composed of a Hartmann-Shack wavefront sensor and a MEMS deformable mirror. The impact of compensating the aberrations of the laser beam is shown in a number of biological and non-biological samples. As examples, nonlinear fluorescence and second harmonic generation images of non-stained ex-vivo ocular tissues are compared with and without adaptive optics. The correction of the beam's aberrations increases both contrast and resolution in the non-linear microscope images.

Citation Download Citation

Emilio J. Gualda, Juan M. Bueno, and Pablo Artal "Adaptive optics multiphoton microscopy", Proc. SPIE 7595, MEMS Adaptive Optics IV, 75950G (18 February 2010); https://doi.org/10.1117/12.845981

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