Quantitative phase microscopy of red blood cells with slightly-off-axis interference

Liang Xue; Jian-cheng Lai; Zhen-hua Li

doi:10.1117/12.870054

8 November 2010 Quantitative phase microscopy of red blood cells with slightly-off-axis interference

Liang Xue, Jian-cheng Lai, Zhen-hua Li

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 784505 (2010) https://doi.org/10.1117/12.870054
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Microscopic interferometry is a noncontact technique for quantitative phase imaging of live cells. The method combines the principles of single-shot slightly-off-axis interferometry and confocal microscopy and is characterized by real-time acquisition capabilities and optimized spatial resolution. However, slightly-off-axis interferometry requires less detector bandwidth than traditional off-axis interferometry and fewer phase-shifted steps than on-axis interferometry. Meanwhile, confocal microscopy allows microstructure magnification imaging. To validate the utility of this technique, experimental and theoretical comparisons are given. The potential of the technique for phase microcopy is demonstrated by experiments on red blood cells. This study will set the basis for interferometric phase measurements of dynamic processes with fine spatial details, especially for observing live biological cell dynamics.

Citation Download Citation

Liang Xue, Jian-cheng Lai, and Zhen-hua Li "Quantitative phase microscopy of red blood cells with slightly-off-axis interference", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784505 (8 November 2010); https://doi.org/10.1117/12.870054

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