Cell flow analysis with a two-photon fluorescence fiber probe

Yu-Chung Chang; Jing Yong Ye; Thommey P. Thomas; James R. Baker Jr.; Theodore B. Norris

doi:10.1117/12.870295

8 November 2010 Cell flow analysis with a two-photon fluorescence fiber probe

Yu-Chung Chang, Jing Yong Ye, Thommey P. Thomas, James R. Baker Jr., Theodore B. Norris

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78450U (2010) https://doi.org/10.1117/12.870295
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

We report the use of a sensitive double-clad fiber (DCF) probe for in situ cell flow velocity measurements and cell analysis by means of two-photon excited fluorescence correlation spectroscopy (FCS). We have demonstrated the feasibility to use this fiber probe for in vivo two-photon flow cytometry previously. However, because of the viscosity of blood and the non-uniform flow nature in vivo, it is problematic to use the detected cell numbers to estimate the sampled blood volume. To precisely calibrate the sampled blood volume, it is necessary to conduct real time flow velocity measurement. We propose to use FCS technique to measure the flow velocity. The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. Our two-photon fluorescence fiber probe has the ability to monitor multiple fluorescent biomarkers simultaneously. We demonstrate that we can distinguish differently labeled cells by their distinct features on the correlation curves. The ability to conduct in situ cell flow analysis using the fiber probe may be useful in disease diagnosis or further comprehension of the circulation system.

Citation Download Citation

Yu-Chung Chang, Jing Yong Ye, Thommey P. Thomas, James R. Baker Jr., and Theodore B. Norris "Cell flow analysis with a two-photon fluorescence fiber probe", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450U (8 November 2010); https://doi.org/10.1117/12.870295

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available