Effect of concentration on photobleaching of hematoporphrin monomethyl ether (HMME) in solutions

Y. Wang; Y. Gu

doi:10.1117/12.868800

8 November 2010 Effect of concentration on photobleaching of hematoporphrin monomethyl ether (HMME) in solutions

Y. Wang, Y. Gu

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78451I (2010) https://doi.org/10.1117/12.868800
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

To analyze the effect of concentration on the photobleaching of HMME, the loss of HMME absorption in simplex solution (PBS) and complex solution (albumin buffer) was monitored using steady-state absorption spectra during 532nm laser irradiation. Three concentrations (4, 10, 80μmol/L) were set for each type solution. Photobleaching curves at each concentration were drawn according to the concentration and time during irradiation. Photobleaching rate coefficients (k) at each concentration were calculated with a mathematical model for singlet oxygen-mediated photobleaching reaction. Then the photobleaching of HMME under different concentrations were compared using the photobleaching rate coefficients (k) and the slope of photobleaching curves. Finally, the effect of concentration on HMME photobleaching was analyzed combining the existence state of HMME at each concentration. The results showed that in both PBS and albumin buffer, the photobleaching rate coefficient of HMME was the largest at 4μmol/L, smaller at 10μmol/L, and the smallest at 80μmol/L. In conclusion, there is a concentration-dependent relationship in the photobleaching rate of HMME, photobleaching will be inhibited when the concentration of HMME increased above certain level at which HMME aggregates formed.

Citation Download Citation

Y. Wang and Y. Gu "Effect of concentration on photobleaching of hematoporphrin monomethyl ether (HMME) in solutions", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451I (8 November 2010); https://doi.org/10.1117/12.868800

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available