An analytic scheme for time-domain diffuse fluorescence tomography in circular geometry: methodology and validation

Jiao Li; Feng Gao; Xin Wang; Limin Zhang; Huijuan Zhao

doi:10.1117/12.868438

8 November 2010 An analytic scheme for time-domain diffuse fluorescence tomography in circular geometry: methodology and validation

Jiao Li, Feng Gao, Xin Wang, Limin Zhang, Huijuan Zhao

Author Affiliations +

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78451W (2010) https://doi.org/10.1117/12.868438
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Near-infrared fluorescence diffuse optical tomography has proven to be an efficient tool for visualizing the bio-distributions of fluorescent markers in tissue. We present a two-dimensional image reconstruction method for time-domain fluorescence diffuse optical tomography on a turbid medium of circular domain. The methodology is based on a linear generalized pulse spectrum technique that employs the analytical solution to the Laplace-transformed time-domain photon-diffusion equation to construct a Born normalized inverse model. A pair of real domain transform-factors is introduced to simultaneously reconstruct the fluorescent yield and lifetime images and the resultant linear inversions are solved using an algebraic reconstruction technique. The algorithm is validated using simulated data, and the spatial resolution, noise-robustness and so on are assessed. The experimental validation is performed using a multi-channel time-correlated single-photon-counting system and a cylinder phantom that embeds a fluorescent target made from 1%-Intralipid solution and Cy5.5 agent. The results show that the approach retrieves the position and shape of the target with a reasonable accuracy.

Citation Download Citation

Jiao Li, Feng Gao, Xin Wang, Limin Zhang, and Huijuan Zhao "An analytic scheme for time-domain diffuse fluorescence tomography in circular geometry: methodology and validation", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451W (8 November 2010); https://doi.org/10.1117/12.868438

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