Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix

Vivide Tuan-Chyan Chang; Delson Merisier; Bing Yu; David K. Walmer; Nirmala Ramanujam

doi:10.1117/12.875855

22 February 2011 Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix

Vivide Tuan-Chyan Chang, Delson Merisier, Bing Yu, David K. Walmer, Nirmala Ramanujam

Author Affiliations +

Proceedings Volume 7891, Design and Quality for Biomedical Technologies IV; 78910A (2011) https://doi.org/10.1117/12.875855
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

A significant challenge in detecting cervical pre-cancer in low-resource settings is the lack of effective screening facilities and trained personnel to detect the disease before it is advanced. Light based technologies, particularly quantitative optical spectroscopy, have the potential to provide an effective, low cost, and portable solution for cervical pre-cancer screening in these communities. We have developed and characterized a portable USB-powered optical spectroscopic system to quantify total hemoglobin content, hemoglobin saturation, and reduced scattering coefficient of cervical tissue in vivo. The system consists of a high-power LED as light source, a bifurcated fiber optic assembly, and two USB spectrometers for sample and calibration spectra acquisitions. The system was subsequently tested in Leogane, Haiti, where diffuse reflectance spectra from 33 colposcopically normal sites in 21 patients were acquired. Two different calibration methods, i.e., a post-study diffuse reflectance standard measurement and a real time self-calibration channel were studied. Our results suggest that a self-calibration channel enabled more accurate extraction of scattering contrast through simultaneous real-time correction of intensity drifts in the system. A self-calibration system also minimizes operator bias and required training. Hence, future contact spectroscopy or imaging systems should incorporate a selfcalibration channel to reliably extract scattering contrast.

Citation Download Citation

Vivide Tuan-Chyan Chang, Delson Merisier, Bing Yu, David K. Walmer, and Nirmala Ramanujam "Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix", Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910A (22 February 2011); https://doi.org/10.1117/12.875855

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