A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging

Delong Zhang; Mikhail N. Slipchenko; Shuhua Yue; Junjie Li; Ji-Xin Cheng

doi:10.1117/12.875886

28 February 2011 A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging

Delong Zhang, Mikhail N. Slipchenko, Shuhua Yue, Junjie Li, Ji-Xin Cheng

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79032L (2011) https://doi.org/10.1117/12.875886
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We demonstrate nonlinear vibrational imaging of isolated Raman bands by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produces a stimulated Raman loss signal that is 12 times larger than what picosecond pulse excitation produces. The strong signal allowed real-time, bond-selective imaging of deuterated palmitic acid-d31 inside live cells, and 3D sectioning of fat storage in live C. elegans. With the high peak power provided by femtosecond pulses, this system is highly compatible with other nonlinear optical modalities such as two-photon excited fluorescence. With most of the excitation power contributed by the Stokes beam in the 1.0 - 1.2 μm wavelength range, photodamage of biological samples was not observed.

Citation Download Citation

Delong Zhang, Mikhail N. Slipchenko, Shuhua Yue, Junjie Li, and Ji-Xin Cheng "A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032L (28 February 2011); https://doi.org/10.1117/12.875886

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