CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 7908 > Article
Paper
11 February 2011 Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids
Kevin Braeckmans, Kevin Buyens, Wim Bouquet-Geerardyn, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, Loïc Doeuvre, Eduardo Angles-Cano, Niek N. Sanders, Jo Demeester, Stefaan C. De Smedt
Author Affiliations +
Proceedings Volume 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 79080B (2011) https://doi.org/10.1117/12.889218
Event: SPIE BiOS, 2011, San Francisco, California, United States
Abstract
While extremely relevant to many life science fields, such as biomedical diagnostics and drug delivery, studies on the size of nanoparticulate matter dispersed in biofluids are missing due to a lack of suitable methods. Here we report that fluorescence single particle tracking (fSPT) with maximum entropy analysis is the first technique suited for accurate sizing of nanoparticles dispersed in biofluids, such as whole blood. After a thorough validation, the fSPT sizing method was applied to liposomes that have been under investigation for decades as nanocarriers for drugs. The tendency of these liposomes to form aggregates in whole blood was tested in vitro and in vivo. In addition, we have demonstrated that the fSPT sizing technique can be used for identifying and sizing natural cell-derived microparticles directly in plasma. fSPT sizing opens up the possibility to systematically study the size and aggregation of endogenous or exogenous nanoparticles in biofluids.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Kevin Braeckmans, Kevin Buyens, Wim Bouquet-Geerardyn, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, Loïc Doeuvre, Eduardo Angles-Cano, Niek N. Sanders, Jo Demeester, and Stefaan C. De Smedt "Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080B (11 February 2011); https://doi.org/10.1117/12.889218
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Blood
Particles
Nanoparticles
Luminescence
Blood circulation
Diffusion
Biomedical optics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top