Rapid and field-deployable biological and chemical Raman-based identification

Edita Botonjic-Sehic; Tracy L. Paxon; Hacene Boudries

doi:10.1117/12.881612

12 May 2011 Rapid and field-deployable biological and chemical Raman-based identification

Edita Botonjic-Sehic, Tracy L. Paxon, Hacene Boudries

Author Affiliations +

Proceedings Volume 8032, Next-Generation Spectroscopic Technologies IV; 80320G (2011) https://doi.org/10.1117/12.881612
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Pathogen detection using Raman spectroscopy is achieved through the use of a sandwich immunoassay. Antibody-modified magnetic beads are used to capture and concentrate target analytes in solution and surface-enhanced Raman spectroscopy (SERS) tags are conjugated with antibodies and act as labels to enable specific detection of biological pathogens. The rapid detection of biological pathogens is critical to first responders, thus assays to detect E.Coli and Anthrax have been developed and will be reported. The problems associated with pathogen detection resulting from the spectral complexity and variability of microorganisms are overcome through the use of SERS tags, which provide an intense, easily recognizable, and spectrally consistent Raman signal. The developed E. coli assay has been tested with 5 strains of E. coli and shows a low limit of detection, on the order of 10 and 100 c.f.u. per assay. Additionally, the SERS assay utilizes magnetic beads to collect the labeled pathogens into the focal point of the detection laser beam, making the assay robust to commonly encountered white powder interferants such as flour, baking powder, and corn starch. The reagents were also found to be stable at room temperature over extended periods of time with testing conducted over a one year period. Finally, through a specialized software algorithm, the assays are interfaced to the Raman instrument, StreetLab Mobile, for rapid-field-deployable biological identification.

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Edita Botonjic-Sehic, Tracy L. Paxon, and Hacene Boudries "Rapid and field-deployable biological and chemical Raman-based identification", Proc. SPIE 8032, Next-Generation Spectroscopic Technologies IV, 80320G (12 May 2011); https://doi.org/10.1117/12.881612

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KEYWORDS

Surface enhanced Raman spectroscopy

Pathogens

Raman spectroscopy

Magnetism

Biological research

Control systems

Spectroscopy

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