Laser Doppler Microscopy Of Living Cytoplasm

Richard P. C. Johnson; Graeme R. A. Dunbar

doi:10.1117/12.941473

10 September 1987 Laser Doppler Microscopy Of Living Cytoplasm

Richard P. C. Johnson, Graeme R. A. Dunbar

Proceedings Volume 0808, Inverse Problems in Optics; (1987) https://doi.org/10.1117/12.941473
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

The use of a laser Doppler microscope, developed to measure flow and/or diffusion in living cells, is described. It analyses Doppler shifts given to the frequency of laser light scattered at a known angle from particles moving in a volume of less than 200 cubic micrometres. This scattering volume is defined in the specimen by an aperture in an image-plane and can be placed within a single cell. Specimens can be viewed and recorded continuously by video-enhanced differential interference contrast microscopy during experiments so that, independently of laser-Doppler measurements, sizes of individual particles in images may be measured and their displacements timed with the aid of a video-micrometer. This paper describes how ways are now needed to constrain and improve computation and analysis of the laser Doppler measurements by incorporating information from the images.

Citation Download Citation

Richard P. C. Johnson and Graeme R. A. Dunbar "Laser Doppler Microscopy Of Living Cytoplasm", Proc. SPIE 0808, Inverse Problems in Optics, (10 September 1987); https://doi.org/10.1117/12.941473

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