Lensfree in-line holographic detection of bacteria

V. Poher; C. P. Allier; J. G. Coutard; L. Hervé; J. M. Dinten

doi:10.1117/12.889324

8 June 2011 Lensfree in-line holographic detection of bacteria

V. Poher, C. P. Allier, J. G. Coutard, L. Hervé, J. M. Dinten

Proceedings Volume 8086, Advanced Microscopy Techniques II; 808619 (2011) https://doi.org/10.1117/12.889324
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Due to low light scattering, bacteria are difficult to detect using lensless imaging systems. In order to detect individual bacteria, we report a method based on a thin wetting film imaging that produces a micro-lens effect on top of each bacterium when the sample dries up. The imaging using a high-end CMOS sensor is combined with an in-line holographic reconstruction to improve positive detection rate up to 95% with micron-sized beads at high density of ~10³ objects/mm². The system allows detecting from single bacterium to densely packed objects (10³ bacteria/μl) within 10μl sample. As an example, E.coli, Bacillus subtilis and Bacillus thuringiensis, has been successfully detected with strong signal to noise ratio across a 24mm² field of view.

Citation Download Citation

V. Poher, C. P. Allier, J. G. Coutard, L. Hervé, and J. M. Dinten "Lensfree in-line holographic detection of bacteria", Proc. SPIE 8086, Advanced Microscopy Techniques II, 808619 (8 June 2011); https://doi.org/10.1117/12.889324

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