Paper
14 June 2011 Detection and visualization improvement of spermatozoa cells by digital holography
L. Miccio, A. Finizio, P. Memmolo, M. Paturzo, F. Merola, G. Coppola, G. Di Caprio, M. Gioffrè, R. Puglisi, D. Balduzzi, A. Galli, P. Ferraro
Author Affiliations +
Abstract
Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
L. Miccio, A. Finizio, P. Memmolo, M. Paturzo, F. Merola, G. Coppola, G. Di Caprio, M. Gioffrè, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro "Detection and visualization improvement of spermatozoa cells by digital holography", Proc. SPIE 8089, Molecular Imaging III, 80890C (14 June 2011); https://doi.org/10.1117/12.889972
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Cited by 6 scholarly publications.
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KEYWORDS
Digital holography

Visualization

Digital image correlation

Holograms

Wavefronts

Microfluidics

Microscopy

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