Optical immunosensor for endocrine disruptor nanolayer detection by surface plasmon resonance imaging

Alina Karabchevsky; Lev Tsapovsky; Robert S. Marks; Ibrahim Abdulhalim

doi:10.1117/12.899617

12 September 2011 Optical immunosensor for endocrine disruptor nanolayer detection by surface plasmon resonance imaging

Alina Karabchevsky, Lev Tsapovsky, Robert S. Marks, Ibrahim Abdulhalim

Proceedings Volume 8099, Biosensing and Nanomedicine IV; 809918 (2011) https://doi.org/10.1117/12.899617
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

Endocrine disrupting compounds (EDCs) such as bisphenol A (BPA) and female hormone Estrone are especially prevalent in surface and waste-waters in nano-molar concentrations and therefore, there is a need for sensitive analytical device for their monitoring. We have designed a miniature, low cost and fast surface plasmon resonance (SPR) imaging liquid sensor based on the angular interrogation using Kretschmann configuration with diverged incident monochromatic light. During this paper we present a surface plasmon resonance imaging (SPRI) biosensor to detect EDCs such as BPA and estrone. A pattern of SPR line which is dark intensity line on bright area was reflected at angles range depending on the dielectric constant of the analye: Rabbit Anti-Estrone polyclonal IgG + Estrone 11-MUA attached to the silver or non-specific sensing of BPA in water with nanoprecision. For analyzing the SPR signals we used an efficient detection algorithm based on Radon Transform with less sensitivity to laser speckle noise and nonuniformity of the illumination.

Citation Download Citation

Alina Karabchevsky, Lev Tsapovsky, Robert S. Marks, and Ibrahim Abdulhalim "Optical immunosensor for endocrine disruptor nanolayer detection by surface plasmon resonance imaging", Proc. SPIE 8099, Biosensing and Nanomedicine IV, 809918 (12 September 2011); https://doi.org/10.1117/12.899617

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