Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Okhil Kumar Nag; Han Young Woo; Wei R. Chen

doi:10.1117/12.901836

14 February 2012 Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Okhil Kumar Nag, Han Young Woo, Wei R. Chen

Author Affiliations +

Proceedings Volume 8224, Biophotonics and Immune Responses VII; 82240G (2012) https://doi.org/10.1117/12.901836
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N-bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

Citation Download Citation

Okhil Kumar Nag, Han Young Woo, and Wei R. Chen "Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant", Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240G (14 February 2012); https://doi.org/10.1117/12.901836

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