Comparsion of light dose on topical ALA-mediated photodynamic therapy for DMBA-induced hamster buccal pouch premalignant lesions

Deng-Fu Yang; Meng-Ke Tseng; Chung-Ji Liu; Yih-Chih Hsu

doi:10.1117/12.907542

14 February 2012 Comparsion of light dose on topical ALA-mediated photodynamic therapy for DMBA-induced hamster buccal pouch premalignant lesions

Deng-Fu Yang, Meng-Ke Tseng, Chung-Ji Liu, Yih-Chih Hsu

Author Affiliations +

Proceedings Volume 8224, Biophotonics and Immune Responses VII; 82240R (2012) https://doi.org/10.1117/12.907542
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Oral cancer has becomes the most prominent male cancer disease due to the local betel nut chewing habit combing with smoking and alcohol-drinking lifestyle. In order to minimize the systemic phototoxic effect of 5-aminolevulinic acid (ALA), this study was designed to use a topical ALA-mediated PDT for treatment of DMBA-induced hamster buccal pouch cancerous lesions. DMBA was applied to one of the buccal pouches of hamsters thrice a week for 8 to 10 weeks. Precancerous lesions were induced and proven by histological examination. These DMBA-induced cancerous lesions were used for testing the efficacy of topical ALA -mediated PDT. We found that ALA reached its peak level in cancerous lesions about 2.5 hrs after topical application of ALA gel. The precancerous lesions in hamsters were then treated with topical ALA -mediated PDT with light exposure dose of 75 and 100 J/cm² using LED 635 nm Wonderlight device. It is suggesting that optimization of the given light dose is critical to the success of PDT results.

Citation Download Citation

Deng-Fu Yang, Meng-Ke Tseng, Chung-Ji Liu, and Yih-Chih Hsu "Comparsion of light dose on topical ALA-mediated photodynamic therapy for DMBA-induced hamster buccal pouch premalignant lesions", Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240R (14 February 2012); https://doi.org/10.1117/12.907542

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