Surface modification of upconversion nanoparticles with amphiphilic chitosan for cancer cell imaging

Sisi Cui; Hongyan Zhu; Haiyan Chen; Junmei Tian; Wei R. Chen; Yueqing Gu

doi:10.1117/12.907916

14 February 2012 Surface modification of upconversion nanoparticles with amphiphilic chitosan for cancer cell imaging

Sisi Cui, Hongyan Zhu, Haiyan Chen, Junmei Tian, Wei R. Chen, Yueqing Gu

Proceedings Volume 8224, Biophotonics and Immune Responses VII; 82240V (2012) https://doi.org/10.1117/12.907916
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Upconversion nanoparticles (UCNPs) as a new kind of biological luminescence materials have many advantages comparing with organic fluorescence probes and semi-conductive quantum dots, such as sharp fluorescence emission, long emission lifetimes, high optical and chemical stability and low toxicity, especially low auto-fluorescence background and deep tissue penetration under near-infrared (NIR) light excitation for bioimaging. Herein, we demonstrate a facile approach to transfer UCNPs from hydrophobic to hydrophilic and the use of these UCNPs for cell imaging. Oleic acid-capped UCNPs based on NaYF₄ were synthesized and modified with amphiphilic chitosan derivative through hydrophobic interaction. The as-prepared chitosan coated UCNPs with an average diameter of 35 nm were mono-dispersed in aqueous solution and possess good optical properties upon NIR light excitation. Cell viability assays indicated the low cytotoxicity and good biocompatibility of chitosan-coated UCNPs. Cell imaging results demonstrated that chitosan-coated UCNPs had great potential for bioimaing and biolabeling. Our work suggests a feasible method to modify OA-UCNPs with amphiphilic polymer and the promise of chitosan-based UCNPs for bioimaging application.

Citation Download Citation

Sisi Cui, Hongyan Zhu, Haiyan Chen, Junmei Tian, Wei R. Chen, and Yueqing Gu "Surface modification of upconversion nanoparticles with amphiphilic chitosan for cancer cell imaging", Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240V (14 February 2012); https://doi.org/10.1117/12.907916

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