Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system

Yair Mega; Joseph Kerimo; Joseph Robinson; Ali Vakili; Nicolette Johnson; Charles DiMarzio

doi:10.1117/12.909534

9 February 2012 Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system

Yair Mega, Joseph Kerimo, Joseph Robinson, Ali Vakili, Nicolette Johnson, Charles DiMarzio

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 822637 (2012) https://doi.org/10.1117/12.909534
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Confocal microscopy can be used as a practical tool in non-invasive applications in medical diagnostics and evaluation. In particular, it is being used for the early detection of skin cancer to identify pathological cellular components and, potentially, replace conventional biopsies. The detection of melanin and its spatial location and distribution plays a crucial role in the detection and evaluation of skin cancer. Our previous work has shown that the visible emission from melanin is strong and can be easily observed with a near-infrared CW laser using low power. This is due to a unique step-wise, (SW) three-photon excitation of melanin. This paper shows that the same SW, 3-photon fluorescence can also be achieved with an inexpensive, continuous-wave laser using a dual-prism scanning system. This demonstrates that the technology could be integrated into a portable confocal microscope for clinical applications. The results presented here are in agreement with images obtained with the larger and more expensive femtosecond laser system used earlier.

Citation Download Citation

Yair Mega, Joseph Kerimo, Joseph Robinson, Ali Vakili, Nicolette Johnson, and Charles DiMarzio "Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822637 (9 February 2012); https://doi.org/10.1117/12.909534

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available