Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu; Nirmal Mazumder; Han-Ruei Tsai; Chih-Wei Hu; Fu-Jen Kao

doi:10.1117/12.907199

13 February 2012 Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu, Nirmal Mazumder, Han-Ruei Tsai, Chih-Wei Hu, Fu-Jen Kao

Proceedings Volume 8228, Single Molecule Spectroscopy and Superresolution Imaging V; 82280C (2012) https://doi.org/10.1117/12.907199
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

Citation Download Citation

Jianjun Qiu, Nirmal Mazumder, Han-Ruei Tsai, Chih-Wei Hu, and Fu-Jen Kao "Stokes vector formalism based second harmonic generation microscopy", Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280C (13 February 2012); https://doi.org/10.1117/12.907199

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