Quantitative determination of the human breast milk macronutrients by near-infrared Raman spectroscopy

Edlene do Carmo M. Motta; Renato A. Zângaro; Landulfo Silveira Jr.

doi:10.1117/12.910438

2 February 2012 Quantitative determination of the human breast milk macronutrients by near-infrared Raman spectroscopy

Edlene do Carmo M. Motta, Renato A. Zângaro, Landulfo Silveira Jr.

Proceedings Volume 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV; 82291F (2012) https://doi.org/10.1117/12.910438
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

This work proposes the evaluation of the macronutrient constitution of human breast milk based on the spectral information provided by near-infrared Raman spectroscopy. Human breast milk (5 mL) from a subject was collected during the first two weeks of breastfeeding and stocked in -20°C freezer. Raman spectra were measured using a Raman spectrometer (830 nm excitation) coupled to a fiber based Raman probe. Spectra of human milk were dominated by bands of proteins, lipids and carbohydrates in the 600-1800 cm^-1 spectral region. Raman spectroscopy revealed differences in the biochemical constitution of human milk depending on the time of breastfeeding startup. This technique could be employed to develop a classification routine for the milk in Human Milk Banking (HMB) depending on the nutritional facts.

Citation Download Citation

Edlene do Carmo M. Motta, Renato A. Zângaro, and Landulfo Silveira Jr. "Quantitative determination of the human breast milk macronutrients by near-infrared Raman spectroscopy", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82291F (2 February 2012); https://doi.org/10.1117/12.910438

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