Probing the interior of synaptic vesicles with internalized nanoparticles

Jennifer C. Gadd; Kristi L. Budzinski; Yang-Hsiang Chan; Fangmao Ye; Daniel T. Chiu

doi:10.1117/12.905091

2 February 2012 Probing the interior of synaptic vesicles with internalized nanoparticles

Jennifer C. Gadd, Kristi L. Budzinski, Yang-Hsiang Chan, Fangmao Ye, Daniel T. Chiu

Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 82320A (2012) https://doi.org/10.1117/12.905091
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Synaptic vesicles are subcellular organelles that are found in the synaptic bouton and are responsible for the propagation of signals between neurons. Synaptic vesicles undergo endo- and exocytosis with the neuronal membrane to load and release neurotransmitters. Here we discuss how we utilize this property to load nanoparticles as a means of probing the interior of synaptic vesicles. To probe the intravesicular region of synaptic vesicles, we have developed a highly sensitive pH-sensing polymer dot. We feel the robust nature of the pH-sensing polymer dot will provide insight into the dynamics of proton loading into synaptic vesicles.

Citation Download Citation

Jennifer C. Gadd, Kristi L. Budzinski, Yang-Hsiang Chan, Fangmao Ye, and Daniel T. Chiu "Probing the interior of synaptic vesicles with internalized nanoparticles", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320A (2 February 2012); https://doi.org/10.1117/12.905091

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