Effects of La0.2Ce0.6Eu0.2F3 nanoparticles capped with polyethylene glycol on human astrocytoma cells in vitro

Nathan J. Withers; Yekaterina Brandt; Antonio C. Rivera; Leisha M. Armijo; Nathaniel C. Cook; Marek Osiński

doi:10.1117/12.911953

2 February 2012 Effects of La_0.2Ce_0.6Eu_0.2F₃ nanoparticles capped with polyethylene glycol on human astrocytoma cells in vitro

Nathan J. Withers, Yekaterina Brandt, Antonio C. Rivera, Leisha M. Armijo, Nathaniel C. Cook, Marek Osiński

Author Affiliations +

Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 82320R (2012) https://doi.org/10.1117/12.911953
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Lanthanide fluoride colloidal nanocrystals offer a way to improve the diagnosis and treatment of cancer through the enhanced absorption of ionizing radiation, as well as providing visible luminescence. In order to explore this possibility, cytotoxicity assays need to be performed on mammalian cells in vitro, to show minimum levels of biocompatibility for future experiments. 20% lanthanum 60% cerium and 20% europium lanthanide fluoride nanocrystals were capped with polyethylene glycol (PEG) of molecular weight 4000 and suspended in deionized water. These nanocrystals were characterized by transmission electron microscopy, muffle furnace ashing, absorbance spectroscopy, dynamic light scattering, and photoluminescence spectroscopy. Visible light microscopy and trypan blue staining was performed on the cells to assay the cytotoxicity of the nanocrystal on the human astrocytoma line U-87 MG, purchased from ATCC.

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Nathan J. Withers, Yekaterina Brandt, Antonio C. Rivera, Leisha M. Armijo, Nathaniel C. Cook, and Marek Osiński "Effects of La_0.2Ce_0.6Eu_0.2F₃ nanoparticles capped with polyethylene glycol on human astrocytoma cells in vitro", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320R (2 February 2012); https://doi.org/10.1117/12.911953

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