Spiral inertial microfluidic devices for continuous blood cell separation

Nivedita Nivedita; Phillip Ligrani; Ian Papautsky

doi:10.1117/12.909936

14 February 2012 Spiral inertial microfluidic devices for continuous blood cell separation

Nivedita Nivedita, Phillip Ligrani, Ian Papautsky

Proceedings Volume 8251, Microfluidics, BioMEMS, and Medical Microsystems X; 82510R (2012) https://doi.org/10.1117/12.909936
Event: SPIE MOEMS-MEMS, 2012, San Francisco, California, United States

Abstract

Enrichment and separation of cell components of blood is critical to clinical diagnostics and therapeutics. Here we report on spiral inertial microfluidic devices which achieve continuous size-based separation of cell mixtures with high throughput. These devices rely on hydrodynamic forces acting on cells within laminar flow, coupled with Dean instability-induced drag arising from the spiral microchannel geometry, to focus cells in streams near the inner channel wall. The spiral devices were optimized to achieve cell separation in less than 8 cm. These improved devices represent an important development because they are not only small in size (<1 in²), but exhibit high separation efficiency (~90%) and high throughput rates up to 1 million cells per minute. These device concepts offer a path towards possible development of a lab-on-chip for blood analysis and reagent free sample preparation, illustrated by the present results, which successfully demonstrate separation of erythrocytes from leukocytes with whole blood.

Citation Download Citation

Nivedita Nivedita, Phillip Ligrani, and Ian Papautsky "Spiral inertial microfluidic devices for continuous blood cell separation", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510R (14 February 2012); https://doi.org/10.1117/12.909936

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available