A novel intravital multi-harmonic generation microscope for early diagnosis of oral cancer

Yu-Hsiang Cheng; Chih-Feng Lin; Ting-Fang Shih; Chi-Kuang Sun

doi:10.1117/12.2001593

19 March 2013 A novel intravital multi-harmonic generation microscope for early diagnosis of oral cancer

Yu-Hsiang Cheng, Chih-Feng Lin, Ting-Fang Shih, Chi-Kuang Sun

Proceedings Volume 8577, Optical Biopsy XI; 85770R (2013) https://doi.org/10.1117/12.2001593
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Oral cancer is one of the most frequently diagnosed human cancers and leading causes of cancer death all over the world, but the prognosis and overall survival rate are still poor because of delay in diagnosis and lack of early intervention. The failure of early diagnosis is due to insufficiency of proper diagnostic and screening tools and most patients are reluctant to undergo biopsy. Optical virtual biopsy techniques, for imaging cells and tissues at microscopic details capable of differentiating benign from malignant lesions non-invasively, are thus highly desirable. A novel multi-harmonic generation microscope, excited by a 1260 nm Cr:forsterite laser, with second and third harmonic signals demonstrating collagen fiber distribution and cell morphology in a sub-micron resolution, was developed for clinical use. To achieve invivo observation inside the human oral cavity, a small objective probe with a suction capability was carefully designed for patients’ comfort and stability. By remotely changing its focus point, the same objective can image the mucosa surface with a low magnification, illuminated by side light-emitting diodes, with a charge-coupled device (CCD) for site location selection before the harmonic generation biopsy was applied. Furthermore, the slow galvanometer mirror and the fast resonant mirror provide a 30 fps frame rate for high-speed real-time observation and the z-motor of this system is triggered at the same rate to provide fast 3D scanning, again ensuring patients’ comfort. Focusing on the special cytological and morphological changes of the oral epithelial cells, our preliminary result disclosed excellent consistency with traditional histopathology studies.

Citation Download Citation

Yu-Hsiang Cheng, Chih-Feng Lin, Ting-Fang Shih, and Chi-Kuang Sun "A novel intravital multi-harmonic generation microscope for early diagnosis of oral cancer", Proc. SPIE 8577, Optical Biopsy XI, 85770R (19 March 2013); https://doi.org/10.1117/12.2001593

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