Multimodal optoacoustic and multiphoton fluorescence microscopy

Gali Sela; Daniel Razansky; Shy Shoham

doi:10.1117/12.2004815

4 March 2013 Multimodal optoacoustic and multiphoton fluorescence microscopy

Gali Sela, Daniel Razansky, Shy Shoham

Proceedings Volume 8581, Photons Plus Ultrasound: Imaging and Sensing 2013; 85812H (2013) https://doi.org/10.1117/12.2004815
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Multiphoton microscopy is a powerful imaging modality that enables structural and functional imaging with cellular and sub-cellular resolution, deep within biological tissues. Yet, its main contrast mechanism relies on extrinsically administered fluorescent indicators. Here we developed a system for simultaneous multimodal optoacoustic and multiphoton fluorescence 3D imaging, which attains both absorption and fluorescence-based contrast by integrating an ultrasonic transducer into a two-photon laser scanning microscope. The system is readily shown to enable acquisition of multimodal microscopic images of fluorescently labeled targets and cell cultures as well as intrinsic absorption-based images of pigmented biological tissue. During initial experiments, it was further observed that that detected optoacoustically-induced response contains low frequency signal variations, presumably due to cavitation-mediated signal generation by the high repetition rate (80MHz) near IR femtosecond laser. The multimodal system may provide complementary structural and functional information to the fluorescently labeled tissue, by superimposing optoacoustic images of intrinsic tissue chromophores, such as melanin deposits, pigmentation, and hemoglobin or other extrinsic particle or dye-based markers highly absorptive in the NIR spectrum.

Citation Download Citation

Gali Sela, Daniel Razansky, and Shy Shoham "Multimodal optoacoustic and multiphoton fluorescence microscopy", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85812H (4 March 2013); https://doi.org/10.1117/12.2004815

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