Adapting phasor analysis for nonlinear pump-probe microscopy

Francisco E. Robles; Jesse W. Wilson; Martin C. Fischer; Warren S. Warren

doi:10.1117/12.2002600

22 February 2013 Adapting phasor analysis for nonlinear pump-probe microscopy

Francisco E. Robles, Jesse W. Wilson, Martin C. Fischer, Warren S. Warren

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 858908 (2013) https://doi.org/10.1117/12.2002600
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Phasor analysis has become a powerful tool for examining signals in fluorescence life-time microscopy (FLIM), where the analysis provides a fast, robust and intuitive means of separating different fluorescent species and mixtures thereof. In this work we adapt this analysis for pump-probe microscopy, a method that provides molecular contrast of pigmented samples by probing their excited state dynamic properties. The bipolar nature of the pump-probe signals presents important differences in the resulting phasors compared to FLIM—here, we discuss these differences and describe the behavior of bipolar signals in phasor analysis. Results show that this method is indeed able to separate multiple molecular species of interests and allows facile assessment of pigment chemistry and its distribution in samples.

Citation Download Citation

Francisco E. Robles, Jesse W. Wilson, Martin C. Fischer, and Warren S. Warren "Adapting phasor analysis for nonlinear pump-probe microscopy", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858908 (22 February 2013); https://doi.org/10.1117/12.2002600

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