Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg; H. I. C. Dalgarno; T. Čižmár; F. J. Gunn-Moore; K. Dholakia

doi:10.1117/12.2003828

22 February 2013 Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg, H. I. C. Dalgarno, T. Čižmár, F. J. Gunn-Moore, K. Dholakia

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 858912 (2013) https://doi.org/10.1117/12.2003828
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

Citation Download Citation

T. Vettenburg, H. I. C. Dalgarno, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia "Increasing the resolution of light sheet microscopy in the presence of aberrations", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858912 (22 February 2013); https://doi.org/10.1117/12.2003828

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available