On alterations in the refractive index and scattering properties of biological tissue caused by histological processing

Htet Aung; Bianca DeAngelo; John Soldano; Piotr Kostyk; Braulio Rodriguez; M. Xu

doi:10.1117/12.2005927

21 February 2013 On alterations in the refractive index and scattering properties of biological tissue caused by histological processing

Htet Aung, Bianca DeAngelo, John Soldano, Piotr Kostyk, Braulio Rodriguez, M. Xu

Author Affiliations +

Proceedings Volume 8592, Biomedical Applications of Light Scattering VII; 85920X (2013) https://doi.org/10.1117/12.2005927
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Clinical tissue processing such as formalin fixing, paraffin-embedding and histological staining alters significantly the optical properties of the tissue. We document the alterations in the optical properties of prostate cancer tissue specimens in the 500nm to 700nm spectral range caused by histological processing with quantitative differential interference contrast (qDIC) microscopy. A simple model to explain these alterations is presented at the end.

Citation Download Citation

Htet Aung, Bianca DeAngelo, John Soldano, Piotr Kostyk, Braulio Rodriguez, and M. Xu "On alterations in the refractive index and scattering properties of biological tissue caused by histological processing", Proc. SPIE 8592, Biomedical Applications of Light Scattering VII, 85920X (21 February 2013); https://doi.org/10.1117/12.2005927

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